Date: 19-20 March 2015
Time: 9:00-16:00
Location: Mike de Vries building, room 2022 (CAF Fluorescent Microscopy Unit)
This course introduces the participant to flow cytometry and the applications of this technology, with a special focus on sorting. Participants will get the chance for hands-on acquisition of samples to familiarise themselves with the work flow on a flow cytometer, the setup and calibration of the instrument and data analysis. Emphasis will be placed on sorting and students will get the chance to run a short sorting experiment themselves.
The content that will be covered include:
• Basic understanding of the components of a flow cytometer
• Knowledge of key concepts: light and colour, scatter, laser delay, pulse area and width, spillover
• Hands on acquisition
• Data analysis using FACSDiva and FlowJo
• Setup and running of a sorting experiment
Participants will receive a certificate of attendance at the end of the course.
Interested students/supervisors should contact Lize Engelbrecht as soon as possible as there are only limited spaces.
If the courses is full, but there is lots of interest, we could repeat the course fairly soon.
