The on-going quest in scientific research to see what could not be seen before has led to the development of extraordinary imaging technology such as Super-Resolution Structured Illumination Microscopy. This technology allows a dramatic increase in the achievable limit of resolution, to visualise structures as small as 100 nm. The important research in the South African context to improve the purity of our water sources, the efficacy of disease treatment and product development on nano-scale, would all benefit from this technology. In contrast with other available super-resolution technologies, the SR-SIM platform is quite flexible in terms of sample preparation and the use of commercially available fluorochromes. With the addition of this high-end confocal microscope to our current collection at Stellenbosch University, South African researchers now have access to high-quality imaging.
Possible Techniques with Fluorescent Microscopy (both Olympus and Confocal instruments)
- Multi-colour and GFP Imaging
- Microorganisms and particle analysis
- Time-lapse Imaging
- Z-sectioning and Co-localization
- Multi-dimensional Imaging
- FRET (Foerster Resonance Energy Transfer)
- Ion Imaging / Ratio Imaging / Ca++ Imaging
- Intensity kinetics
Including the abovementioned techniques, Carl Zeiss Confocal LSM 780 Elyra S1 with SR-SIM superresolution microscope will be able to perform DIC microscopy and the more complicated F-techniques (FRET, FRAP, FCS, and RICS). This would not only allow the determination of the precise spatial location of single large molecules, but also improved quantification of cell dynamics, such as protein-protein interaction and protein mobilisation.
For instrument specifics, click here Specification sheet Carl Zeiss LSM780 with ELYRA S.1. For bookings or more information contact Mrs Lize Engelbrecht.