The Zeiss Axio Observer 7 Inverted Microscope is a platform for either light and/or fluorescence microscopy imaging to analyse fixed cells or tissue sections. An added feature to this particular unit is an incubation chamber, offering the option of performing live cell imaging under control physiological conditions.
The Zeiss Axio Observer 7 Inverted Microscope with incubation chamber is housed at the Tygerberg campus of Stellenbosch University
Zeiss Axio Observer 7 Inverted Microscope performance characteristics
- Calibri LED light source for excitation
- A set up of 7 illumination wavelengths and filters to detect all the main fluorophores such as DAPI, Hoechst, GFP, Alexafluor 488, CFP, YFP, Texas Red, Alexafluor 647 and many more.
- Additional BP filters for FRET experiments using CFP and YFP
- Monochrome camera for fluorescence imaging
- Colour camera for histological imaging
- Brightfield, Dark field, Phase contrast and DIC modalities
- 5x, 10x, 20x, 40x objectives for imaging through culture plates
- 40x (water/glycerol immersion) and 63x (oil immersion) for imaging through cover slips
- Samples can be viewed mounted to a slide or in suspension in formats such as petri dishes or any multi-well plates
- Live Imaging: Incubation chamber with settings to control temperature, CO2/O2 and humidity levels
- ZEN imaging software
Observe cellular dynamics (e.g. chemotaxis) in real time with the live imaging option
Quantify molecular interaction with dynamic FRET experiments using CFP and YFP tagged proteins
Examine histological samples prepared from tissue collected from various source (e.g. brain tumor from mice, human intestinal samples stained to detect H. pylori)
Investigate specific cellular parameters by fluorescent imaging (i.e. macrophage with internalised bacteria)
For a detailed list of specifications see Zeiss AxioObserver Specification Sheet.
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