Flow cytometry is one of the most powerful techniques for characterisation of properties of cells and particles. This technology allows for the rapid and concurrent measurement of the physical and fluorescent parameters of 0.5-50µm particles, as they pass through a laser beam, giving information about the relative size, internal complexity and fluorescent intensity of the particles or cells of interest. The CAF imaging unit is equipped with the BD FACSAria™ IIu flow cytometer, a fixed alignment benchtop cell sorter, which was upgraded from the previous model. This high performance instrument is fitted with a solid-state sapphire laser (488nm), HeNe laser (633nm) and violet laser (405nm). This instrument at our unit allows for single particle acquisition of up to 70 000 events per second and is set up for the simultaneous measurement of up to 8 fluorescent colours and two scatter parameters (size and internal complexity) of cells or particles, making it well-suited for multiparameter experiments. The BD FACSAria™ IIu has widely used applications; including subset identification, analysis of marker expression, cell-cell interactions, cytokine production, cell viability and apoptosis assays. One of its main features is its ability to sort particles or cells of interest by the use of electronic deflection. This instrument is well suited for isolating cells or particles in order to obtain a purified yield of the marker or particle of interest.